Fig. 7
Comparative SDS-PAGE analysis of the digestion of whole human plasma by InpA and proteinase K. Whole human plasma (60 μg total protein) was incubated overnight with InpA and proteinase K (Sigma-Aldrich; product number P2308) at the indicated concentrations (μg ml−1) at 37 °C in PBS, pH 7.4 containing 3 mM DTT. Last lane of each gel was loaded with 10 μg of either InpA or proteinase K only. Samples were prepared and gels stained as in Fig. 6