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Fig. 10 | BMC Microbiology

Fig. 10

From: Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia

Fig. 10

Primary sequence showing all the cleavage sites inflicted during incubation of bovine serum albumin with InpA. Albumin (16 μM) was incubated at 37 °C with InpA (100 μg ml−1) in 10 mM Tris buffer, pH 7.5, in the presence or absence of 2mM DTT, and the incubation mixture sampled over time subjected to MALDI-TOF mass spectrometry. InpA scission sites exclusive to reducing () , non-reducing (), and to both incubation conditions () are indicated. See text for details

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