Fig. 1From: Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia Effect of dithiothreitol (a) and other thiol reducing agents (b) on the enzyme activity of InpA. The enzyme activity was assayed in triplicate using Z-Arg-Arg-AMC at 37 °C in 0.1M Tris–HCl, pH 7.5 and the concentrations of the reducing agents in Fig. 1b were 3 mM. Due to the high reproducibility of the replicates, no SD is apparent. Control, activity in the absence of reducing agent. InpA concentrations in (a) and (b) were 12.5 μg ml−1. See text for detailsBack to article page