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Fig. 1 | BMC Microbiology

Fig. 1

From: Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia

Fig. 1

Effect of dithiothreitol (a) and other thiol reducing agents (b) on the enzyme activity of InpA. The enzyme activity was assayed in triplicate using Z-Arg-Arg-AMC at 37 °C in 0.1M Tris–HCl, pH 7.5 and the concentrations of the reducing agents in Fig. 1b were 3 mM. Due to the high reproducibility of the replicates, no SD is apparent. Control, activity in the absence of reducing agent. InpA concentrations in (a) and (b) were 12.5 μg ml−1. See text for details

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