Fig. 3

Pathological analysis of infected newborn mice after intracerebral challenge with a lethal dose (10^6.5 CCID₅₀/ml) of EV-A71CC063. One-day-old ICR mice were intracerebrally inoculated with EV-A71CC063 (10^6.5 CCID₅₀/ml) or medium (mock control). Representative images from the hind-limb muscle, spine muscle, cardiac muscle, and lung tissue at 6 days post-infection are shown. Infected mice (clinical grades 4-5) exhibited severe necrosis in the hind-limb muscle (b) and spinal skeletal muscle (d), but no obvious histological changes in cardiac muscle (f) or lung tissue (h) by HE staining. The results for non-infected mice were used as a control (a, c, e, and g). Magnification, 400 ×