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Table 1 Analysis of autophagy in control (Ws54-1255 (GFP-SKL)) and Δatg1 (GFP-SKL) strains

From: Penicillium chrysogenum as a model system for studying cellular effects of methylglyoxal

 

20 h

40 h

60 h

Control

-

+

++

+ MG

-

-

+

+ R

-

++

+++

+ MG/+ R

-

(+)

+

Δatg1

-

-

-

+ MG

-

-

-

+ R

-

-

-

+ MG/+ R

-

-

-

  1. For each data point around 100 hyphae were analysed by fluorescence microscopy. -: no peroxisome degradation observed, (+): weak peroxisome degradation (5–10 % of all hyphae), +: ca. 25 % of hyphae display peroxisome degradation, ++: ca. 50 % of hyphae display peroxisome degradation, +++: 90–100 % of hyphae display peroxisome degradation.MG methylglyoxal (0.05 % [v/v]). R rapamycin (1 μM)