Skip to main content
Fig. 5 | BMC Microbiology

Fig. 5

From: Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231

Fig. 5

Size exclusion profile of the purified HP0377 separated on a Superdex75 HiLoad 16/600 column (GE Healthcare) and monitored by absorbance at 280 nm. HP0377 elutes as a two peaks at 61 min, with an estimated mass of 48 kDa, consistent with the size of the homodimer and 66 min. with estimated mass of 24 kDa, consistent with the size of the monomer. The column was calibrated with Gel Filtration Standard (Bio-Rad): Thyroglobulin (670 kDa), γ-globulin (158 kDa), Ovalbumin (44 kDa), Myoglobin (17 kDa), Vitamin B12 (1.35 kDa). The relative positions of the chosen standards are marked with arrows

Back to article page