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Fig. 1 | BMC Microbiology

Fig. 1

From: Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231

Fig. 1

Redox state of HP0377 in wt and mutants: hp0231::cat, dsbI::aph and complemented strains of H. pylori strain N6. Bacterial cultures were treated with 10 % TCA, followed by alkylation with AMS. Cellular proteins including the reduced (red; DTT treated, modified with AMS) and the oxidized (ox; non-modified with AMS) controls were separated by 14 % SDS-PAGE under non-reducing conditions, and Western blot analysis antibodies against HP0377 was performed. Each lane contains proteins isolated from the same amount of bacteria

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BMC Microbiology

ISSN: 1471-2180

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