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Table 2 Growth of C. glutamicum WT and ΔphoRS on different phosphorus sources

From: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Phosphorus source

Strain

Biomass formed [g/l]

μ [h−1]

Duration of lag phase [h]

UDP-glucose hydrolase activity in supernatants [nmol min−1 ml−1] a

Low Pi, 0.065 mMb

WT

2

0.14

0

27

 

ΔphoRS

1

0.07

6

39

Glycerol-3-phosphate, 1 mM

WT

11

0.16

0

9

 

ΔphoRS

9

0.11

9

13

5’AMP, 1 mM

WT

9

0.08

11

6

 

ΔphoRS

7

0.08

34

12

UDP-glucose, 1 mM

WT

9

0.06

39

6

 

ΔphoRS

8

0.09

63

3

  1. aUDP-glucose hydrolase activity was measured after 180 h of cultivation. No UDP-glucose hydrolase activity was detectable (<1 nmol min−1 ml−1) in supernatants of cells grown under Pi sufficient conditions (13 mM)
  2. bThis concentration is below the Pi concentration of 0.1 mM which supports the half-maximal growth rate in C. glutamicum [31]