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Table 1 Strains and plasmids used in this study

From: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Strain or plasmid

Relevant characteristic

Reference

C. glutamicum

  

WT

wild type strain ATCC 13032

[9]

ΔphoRS

deletion of the phoRS operon encoding the two component system PhoRS

[33]

ΔramB

Deletion of ramB encoding regulator of acetate metabolism B

[41]

E. coli

  

BL21(DE3)

ompT hsdS B(rBmB) gal dcm (DE3)

[64]

DH5α

F thi-1 endA1 hsdr17(r, m) supE44 ΔblacU169 (ф80lacZΔM15) recA1 gyrA96 relA1

[65]

Plasmids

  

pGEM-T

cloning vector

Promega, WI, USA

pET2

promoter-probe vector

[54]

pET2-RF0

pET2 with pstSCAB promoter fragment RF0

This study

pET2-R0F0

pET2 with pstSCAB promoterfragment R0F0

This study

pET2-R1F0

pET2 with pstSCAB promoter fragment R1F0

This study

pET2-R2F0

pET2 with pstSCAB promoter fragment R2F0

This study

pET2-R3F0

pET2 with pstSCAB promoter fragment R3F0

This study

pET2-R0F1

pET2 with pstSCAB promoter fragment R0F1

This study

pET2-R0F2

pET2 with pstSCAB promoter fragment R0F2

This study

pET2-R0F3

pET2 with pstSCAB promoter fragment R0F3

This study

pET2-RcFc

pET2 with pstSCAB promoter fragment RcFc

This study

pET2-RcFm

pET2 with pstSCAB promoter fragment RcFm

This study

pET2-RmFc

pET2 with pstSCAB promoter fragment RmFc

This study

pET2-RmFm

pET2 with pstSCAB promoter fragment RmFm

This study

pET29-ramB-his

KanR; pET29-Histag derivative for over production of RamB with a C-terminal histidine tag

[41]