Fig. 3
SDS-PAGE images of DNA affinity chromatography experiment and purified RamB protein. (a) Proteins eluted from a DNA affinity chromatography experiment using the pstS promoter. For the DNA affinity chromatography experiment, the pstS promoter fragment R0F0 was used as a probe and incubated with cell extracts of C. glutamicum ΔphoRS grown under Pi sufficient conditions in minimal medium with 4 % (w/v) glucose (Right lane). 1: DNA-polymerase I, 2: Acetyl/propionyl-CoA carboxylase subunit, 3: Acetyl/propionyl-CoA carboxylase subunit, 4: DNA gyrase, 5: DNA-directed RNA polymerase β-subunit, 6: DNA-directed RNA polymerase β’-subunit. Left lane: protein standard Seeblue II prestained Standard (Invitrogen, Karlsruhe) (b) Purified His-tagged RamB. His-tagged RamB was over produced in E. coli, purified and separated on a 10 % (w/v) SDS-polyacrylamide gel. Gel was stained with Coomassie Blue. Left lane: protein standard Seeblue II prestained Standard (Invitrogen, Karlsruhe), Right lane: purified His-tagged RamB obtained after imidazol elution from a nickel-chelate affinity column