Extensive characterization of Campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization

Table 2 PCR conditions for the detection of the genes specific to the strains used in the in vivo chicken colonization competition assay

Gene	Primers (5′–3′)	Cycle
pseD	F: GATGGAATGGTAAGCGTTGCACA	95°C for 20 s
	R: TTATCCTTGCTCCACCTTCGGTGC	56°C for 20 s
		72°C for 20 s
virB11	F: TCTTGTGAGTTGCCTTACCCCTTTT	95°C for 20 s
	R: CCTGCGTGTCCTGTGTTATTTACCC	65°C for 20 s
		72°C for 20 s
CJ0144	F: TTCATGAAGTTGTGAATGCTGAAA	95°C for 20 s
	R: TCGCTTACAACAAGTTCGCC	61°C for 20 s
		72°C for 10 s

All PCR cycles were preceded by an initial denaturation step consisting of a 15-min incubation at 95°C; All PCR runs were ended by a final 5-min elongation step at 72°C followed by a denaturation curve analysis.

ISSN: 1471-2180