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Table 3 Bacterial viability determination from untreated and heat-fixed kidney tissue obtained from a tissue mimetic model

From: Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging

Bacterial isolate

Treatment

1:10

1:100

1:1000

cfu/ml

E.coli Top10

none

TNTC

1000+

1000+

>103

E.coli pGSV3-GmR

none

TNTC

1000+

1000+

>103

E.coli pAM17-KanR

none

TNTC

1000+

1000+

>103

Klebsiella pneumoniae

none

TNTC

1000+

1000+

>103

Burkholderia thailandiensis

none

TNTC

1000+

1000+

>103

Acinetobacter baumani

none

TNTC

1000+

1000+

>103

Control (no bacteria)

none

0

0

0

0

E.coli Top10

heat fixed

0

0

0

0

E.coli pGSV3-GmR

heat fixed

0

0

0

0

E.coli pAM17-KanR

heat fixed

0

0

0

0

Klebsiella pneumoniae

heat fixed

0

0

0

0

Burkholderia thailandiensis

heat fixed

0

0

0

0

Acinetobacter baumani

heat fixed

0

0

0

0

Control (no bacteria)

heat fixed

0

0

0

0

  1. TNTC = too numerous to count, HF = Heat fixed.