Figure 4

Visualization of Tfp during movement using TIRF microscopy. Live bacteria were stained with the Dylight™488 NHS-ester prior to experiments. (A) Representative pictures of stained N. gonorrhoeae and N. meningitidis crawling on a poly-D-lysine-coated surface. The scale bar corresponds to 5 μm. (B) Percent of observations with x number of visible filaments during movement on a solid surface. More than 2,600 individual frames from the time-lapse movies were analyzed for each species, and the number of visible filaments was counted in each frame.