Figure 3
From: Characterization of motility and piliation in pathogenic Neisseria
Analysis of PilT expression in pilT promoter mutants. The pilT promoter constructs used to transform N. gonorrhoeae N400 and N. meningitidis FAM20 carried either the 5’TATAAT or the 5’TACAAT sequence with a chloramphenicol resistance cassette (CmR) oriented on the opposite strand and in the opposite direction of the pilT gene. pilT mRNA transcription level in N400 (A) and FAM20 (B), as quantified using qPCR. mRNA expression was normalized to three different reference genes (i.e., 16S rRNA, 50S ribosomal protein rplP and σ factor rpoD) and compared to the WT level. The experiment was performed two to three times. The bars show the mean ± standard deviation. PilT protein quantification of whole cell lysates from N400 (C) and FAM20 (D) by western blot. The relative PilT/EF-Tu band intensities were averaged over two to four separate blots and normalized against WT strains. The error bars represent the standard deviation.