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Figure 2 | BMC Microbiology

Figure 2

From: Characterization of motility and piliation in pathogenic Neisseria

Figure 2

Analysis of PilT expression in N. gonorrhoeae strain FA1090 and N. meningitidis strain FAM20. (A) Bar chart showing average pilT mRNA levels in FA1090 and FAM20 normalized to 16S rRNA, 50S ribosomal gene rplP and σ factor rpoD. The error bars represent the standard deviation. The experiment was performed twice. (B) Representative immunoblots and PilT protein quantification. Bacterial lysates were separated on a 12% SDS-PAGE gel, probed with an anti-PilT antibody and an anti-EF-Tu antibody, and analyzed using an IR-scanner. EF-Tu migrates at 45 kDa, and PilT migrates at 37 kDa. The relative PilT/EF-Tu band intensities were averaged over more than three separate blots and normalized against FA1090. The error bars represent the standard error. Significant differences are marked with an asterisk * (p < 0.05 using an unpaired two-tailed Student’s t-test). (C) Alignment of the pilT-promoter region from sequenced gonococcal and meningococcal genomes. Dots indicate sequences identical to the reference FA1090 sequence, and sequence differences are highlighted. The boxes indicate the −35 and −10 promoter regions. The arrow denotes the main pilT-mRNA, and the dashed box indicates the pilT start codon.

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