Figure 5

Phylogenetic relationships of the V. vulnificus AcsR (A) and AcsS (B) proteins. The pairwise phylogenetic distances were calculated using the Poisson correction method, and the tree was constructed using the Neighbor-Joining algorithm implemented in MEGA6 (software). The numbers at the nodes indicate the bootstrap score as percentage and are shown for frequencies at or above the threshold of 50%. The scale bar represents the expected number of substitutions per amino acid position. Sequences obtained from GenBank included Vibrio vulnificus (YP_004187421.1), V. parahaemolyticus (WP_025522838.1), V. cholera (WP_000378642.1), V. alginolyticus (EMD77453.1), V. harveyi (EEZ86880.1), V. splendidus (WP_019821543.1), Aliivibrio fischeri (WP_011262765.1), Shewanella oneidensis (WP_011072594.1), Pseudomonas syringae (WP_032610538.1), P. aeruginosa (NP_252294.1), Brucella melitensis (NP_540524.1), Escherichia coli (WP_032358651.1), Bacillus subtilis (WP_014481054.1), Staphylococcus aureus (WP_001045137.1), Enterococcus faecium (WP_029485364.1), Listeria monocytogenes (WP_012951438.1) for AcsR analysis. Information on the AcsS proteins included V. vulnificus (YP_004187416.1), V. parahaemolyticus (NP_799253.1), V. cholera (NP_229958.1), V. alginolyticus (WP_005397851.1), V. harveyi (WP_005440411.1), V. splendidus (WP_032497689.1), A. fischeri (WP_012533517.1), S. oneidensis (NP_718326.1), P. syringae (KFE45115.1), P. aeruginosa (WP_023464967.1), B. melitensis (WP_004685428.1), E. coli (WP_032225459.1), B. subtilis (WP_003237770.1), S. aureus (WP_000975271.1), E. faecium (BAM68246.1), L. monocytogenes (WP_031646040.1).