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Table 2 Relative expression of genes involved in osteogenic differentiation

From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

 

Day 3

Day 7

 

Relative expression

Fold difference

Relative expression

Fold difference

ALP

    

  Differentiation control

13.11 ± 0.34

7.51

7.69 ± 0.51

14.55

  S. aureus UAMS-1

3.23 ± 0.25

1.85

0.27 ± 0.38

0.52

  P. aeruginosa SAMMC-418

0.53 ± 0.35

0.30

0.07 ± 0.45

0.13

RUNX2

    

  Differentiation control

0.53 ± 0.82

3.54

0.91 ± 0.72

2.59

  S. aureus UAMS-1

0.21 ± 0.99

1.42

1.24 ± 0.70

3.56

  P. aeruginosa SAMMC-418

0.13 ± 0.62

0.85

1.35 ± 0.67

3.86

OCN

    

  Differentiation control

0.34 ± 0.36

1.92

31.91 ± 0.53

10.42

  S. aureus UAMS-1

0.01 ± 0.35

0.08

0.01 ± 0.86

0.00

  P. aeruginosa SAMMC-418

0.02 ± 0.27

0.12

0.00 ± 0.52

0.00

  1. ALP – alkaline phosphatase; RUNX2 – Runt-related transcription factor 2; OCN – bone gamma-carboxyglutamic acid-containing protein (osteocalcin).
  2. Values represent the mean ± SD relative expression of genes as determined by the 2-ΔCt method. Fold difference is the expression of normalized values as a ratio to undifferentiated growth controls.
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BMC Microbiology

ISSN: 1471-2180

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