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Figure 3 | BMC Microbiology

Figure 3

From: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion

Figure 3

Soluble factors from biofilms differentially affect adipogenic and osteogenic differentiation of hBMSCs in vitro. A) Light microscopy images of hBMSCs stained with Oil Red O (red, 40x magnification) after adipogenic differentiation on days 7, 14 and 21 in the presence of BCM (25%) from S. aureus or P. aeruginosa. B) Quantification of Oil Red O stain (OD500nm) to detect intracellular oil droplets following adipogenic differentiation determined by extraction and solubilization of the dye. C) Western blot analysis of adipogenic proteins: adiponectin, peroxisome proliferator-activated receptor gamma (PPARγ), and leptin. D) Light microscopy images of hBMSCs stained with Alizarin Red S (red, 40x magnification) after osteogenic differentiation on days 7, 14 and 21 in the presence of BCM (25%) from S. aureus or P. aeruginosa. E) Quantification of Alizarin Red S stain (OD415nm) to detect intracellular calcium deposits following osteogenic differentiation. F) Western blot analysis of osteogenic proteins: Runt-related transcription factor 2 (RUNX2), bone gamma-carboxyglutamic acid-containing protein (osteocalcin), and alkaline phosphatase. Quantification results are expressed as a ratio of non-differentiated growth controls (dashed line) at each timepoint; *p < 0.05 compared to differentiation control at each respective timepoint.

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