Figure 5

The impact of elevated AcP levels on DegU ~ P regulated processes. (A) Schematic diagram of the Pta-AckA pathway (B) Colony morphology of 3610 (wild-type), degU (NRS1314), ackA (NRS4770), ackA degS (NRS4771) and ackA degS pgsB (NRS4819). Pictures were taken after 16 h incubation at 37°C. (C) Photographs of swarm expansion plates taken after 6 h of incubation at 37°C. Shown are 3610 (wild-type), Δhag (DS1677), degS (NRS1358), ackA (NRS4770), ackA degS (NRS4771). (D) Photographs of swim expansion plates taken after 16 h incubation at room temperature. Shown are 3610 (wild-type), Δhag (DS1677), degS (NRS1358), ackA (NRS4770) and ackA degS (NRS4771). (E) Exoprotease production of 3610 (wild type strain), degS (NRS1358), ackA (NRS4770) and ackA degS (NRS4771) grown on LB agar plates containing 1.5% milk. Plates were imaged after 16 h incubation at 37°C.