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Figure 2 | BMC Microbiology

Figure 2

From: Lactobacillus paracasei CNCM I-4034 and its culture supernatant modulate Salmonella-induced inflammation in a novel transwell co-culture of human intestinal-like dendritic and Caco-2 cells

Figure 2

Effects of L. paracasei CNCM I-4034 and its cell-free culture supernatant on the secretion of pro-inflammatory cytokines by intestinal-like human dendritic cells through an epithelial layer. The production of IL-1β (Panel A), IL-6 (Panel B), IL-8 (Panel C), IL-12 (p40) (Panel D) and IL-12 (p70) (Panel E) was measured. DCs and Caco-2 cells were incubated for 4 h with the live probiotic L. paracasei or its supernatant, Salmonella, or both and further incubated for 20 h in medium containing antibiotics. The culture supernatants were collected from the lower chamber, and the cytokine levels in the supernatants were determined by immunoassay. The data shown are the mean and SEM of the values obtained in three different experiments. *, p<0.05 compared with controls; #, p<0.05 compared with S.typhi.

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