Table 1 Plasmids and strains used in the study

P. fluorescens

MFE01

Air isolate, Rif^R

[31]

MFE01∆hcp2

MFE01 with early stop codon in hcp2

[31]

MFE01∆hcp1

MFE01 with hcp1 disruption

This study

MFE01∆hcp1 + hcp1

MFE01∆hcp1 with pPSV35 carrying the wild-type hcp1 gene

This study

MFE01∆hcp1∆hcp2

MFE01 with hcp1 and hcp2 mutations

This study

MFE01∆tssC

MFE01 with tssC disruption

This study

MFN1032

Clinical strain

[33]

MFP05

Skin isolate

[32]

Pf-5

Plant isolate

[34]

MFE07

Air isolate

This study

Escherichia coli

DH5αmcr

General cloning strain

LMSM collection

S17.1

RP4-2-Tc::Mu, aph::Tn7, recA, SmR, donor strain for conjugation

[48]

Vectors

pPSV35

P. aeruginosa oriV, lacIq mob+, PlacUV5, pUC18 MCS, expression vector, Gm^R

[49]

pSMC21-gfp

Replicative plasmid, KmR, gfp

[46]

pME3087

Suicide plasmid, Tc^R

[47]

pUC19

Replicative plasmid, lacZ, Ap^R

Invitrogen®