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Figure 2 | BMC Microbiology

Figure 2

From: A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium

Figure 2

Ability of C. crescentus to grow and survive under hyperosmotic stress. A) Growth curves and survival rates of C. crescentus in synthetic medium before and after challenge with different concentrations of sodium chloride and sucrose. After reaching an optical density of 0.7 (OD 600 nm), cells were stressed with sodium chloride or sucrose at concentrations ranging from 40/50 to 200 mM. To measure cell viability, aliquots were removed immediately before and 4h and 8h after exposure to stress condition. Values correspond to the fraction of surviving cells under stress relative to that determined immediately before exposure to stress. Data are mean values of three independent experiments; bars represent the standard error. B) Cell morphology under hyperosmotic stress. Exponentially growing cells (OD 600 nm = 0.7) were challenged with either 60 mM NaCl or 110 mM sucrose, and phase contrast micrographic images were obtained from cultures kept under these conditions for 24 h. A non-stressed culture was used as control.

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