Figure 3

In vivo and in vitro permeability measurements. For in vivo measurements of gut permeability, animals were orally gavaged with FITC-dextran (A) (n = 16 mice per group). For in vitro measures, sections of colon were mounted in Ussing chambers and ⁵¹Cr-EDTA flux (% Hot Sample/hr/cm²) (B) and tissue conductance (mS/cm²) (C) were measured (n = 6 mice per group). Control non-inflamed (EtOH-PBS), control inflamed (DNBS-PBS), bacteria-free culture medium (DNBS-LYBHI), F. prausnitzii strain A2-165 (DNBS-Fprau), and F. prausnitzii A2-165 SN (DNBS-SN) groups. Experiments were performed at least in duplicate. Comparisons involved the non-parametric Kruskal-Wallis test was used followed by a Dunn’s Multiple Comparison test. *p < 0.05 vs. DNBS-PBS, +p < 0.05.