Figure 2

Amplification by PCR of shared unique genes to K. variicola and K. pneumoniae . A) Amplification of shared unique genes to K. variicola using the genomes of control strains such as K. pneumoniae ATCC 13883, K. variicola ATCC BAA-830 T, R. terrigena ATCC 33257, R. platicola ATCC 33531 and K. oxytoca ATCC 49134. B) Amplification of shared unique genes to K. pneumoniae using the genomes of control strains described above. Lane 1, ϕX174/Hae III; Lane 2, mtnC gene (KmtnC-F and -R oligonucleotides); Lane 3, phosphoglycerate mutase gene (KV770-F and -R oligonucleotides); Lane 4, thiopurine S-methyltransferase gene (KV1000-F and -R oligonucleotides); Lane 5, N-acetyltransferase gene (KV1615-F and -R oligonucleotides); Lanes 1 to 6, rpoB gene (CM7 and rpoB-M) in combination with oligonucleotides of shared unique genes K. variicola; Lane 7, rpoB oligonuclotides without DNA (CM7 and rpoB-M); Lane 8, Transferase gene (KP878-F and R oligonucleotides); Lane 9, phosphohydrolase gene (KP888-F and R oligonucleotides) (Table 1).