Figure 5

Live-cell imaging analysis to track the movement of B. thailandensis E264. (A) B. thailandensis E264 (BtE264); BtE264 exposed to cell-free supernatants from: (B), B. pseudomallei 1710a; (C), B. pseudomallei K96243 and (D), B. thailandensis E264. Twenty cells were randomly tracked for movement by light microscopy and their paths of movement were determined from video recording using an ImageJ program (http://rsb.info.nih.gov/ij/). Colored lines show the paths of different cells over a 20 sec period. (E) Motility distance of B. thailandensis untreated BtE264 (non-exposed control) and after exposure to cell-free supernatants from B. pseudomallei 1710a, B. pseudomallei K96243 and B. thailandensis E264 control. (F) Expression of fliC RNA and 23S rRNA of B. thailandensis E264 exposed to cell-free supernatant of B. pseudomallei. RT-PCR of fliC RNA (lane 1–4) and 23S rRNA (lane 8–11) of B. thailandensis E264 colony exposed to cell-free supernatant of B. pseudomallei 1710a (lane 1 and 8), B. pseudomallei K96243 (lane 2 and 9), B. thailandensis E264 (lane 3 and 10), and non-exposed control (lane 4 and 11). Lanes 5 and 12 are PCR of genomic DNA controls for fliC and 23S rRNA primers respectively. Lanes 6 and 13 are no RT negative controls.