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Figure 4 | BMC Microbiology

Figure 4

From: Competition between Burkholderia pseudomallei and B. thailandensis

Figure 4

Inhibitory activities on B. thailandensis E264 motility on swarm agar of cell-free supernatant of B. pseudomallei 1710a after filtration, protein digestion, or incubation under different conditions. (A) Inhibition zone in retentate and filtrate fractions following filtration through membranes with different molecular weight cut-off sizes. (B) Inhibitory activity of cell-free supernatant of B. pseudomallei 1710a after treatments with proteinase K (left panel) and pronase (right panel), (a) no treatment control, (b) treatment with proteinase K or pronase, (c) Enzymes alone (no supernatants) were spotted onto the agar in the same concentration as a control. (C) Inhibition activity of cell-free supernatant of B. pseudomallei 1710a following incubation at different pH at 37°C for 24 h. (D) Inhibition activity of cell-free supernatant of B. pseudomallei 1710a following incubation at different temperatures for 24 h. (E) Inhibition activity of cell-free supernatant of B. pseudomallei 1710a following incubation at different salt concentrations at 37°C for 24 h. The controls for (A), (C), (D) and (E) are B. thailandensis E264 swarming without the inoculation of cell-free supernatant. Plates were incubated at 37°C for 24 h.

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