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Table 1 Kinin-like peptides in the Sap-treated LK samples that were identified using LC-MS/MS

From: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

Sap

Kinin-like peptide

Molecular mass (Da)

Sap1

MKRPPGFSPFR

1318.8

 

MKRP(POH)GFSPFR

1334.7

 

(MO)KRPPGFSPFR

1334.7

 

MKRPPGFSPF

1162.8

 

RPPGFSPFR

1059.8

 

PPGFSPFR

903.4

Sap2

MKRPPGFSPFR

1318.8

 

MKRP(POH)GFSPFR

1334.7

 

(MO)KRPPGFSPFR

1334.7

 

RPPGFSPFR

1059.8

 

LMKRPPGFSPFR

1431.9

 

MKRPPGFSPF

1162.8

 

RP(POH)GFSPF

919.7

Sap3

MKRPPGFSPFR

1318.7

 

MKRP(POH)GFSPFR

1334.7

 

(MO)KRPPGFSPFR

1334.7

 

(MO)KRP(POH)PGFSPFR

1350.7

 

RPPGFSPFR

1059.8

 

LMKRPPGFSPFR

1432.0

 

LMKRP(POH)GFSPFR

1447.8

 

L(MO)KRPGFSPFR

1447.8

Sap4

MKRPPGFSPFR

1318.7

 

MKRP(POH)GFSPFR

1334.7

 

(MO)KRPPGFSPFR

1334.7

 

(MO)KRP(POH)PGFSPFR

1350.7

 

RPPGFSPFR

1059.8

 

RP(POH)GFSPF

919.7

Sap5

MKRPPGFSPFR

1318.8

 

RPPGFSPFR

1059.8

Sap6

MKRPPGFSPFR

1318.8

 

RPPGFSPFR

1059.8

 

MKRP(POH)GFSPFR

1334.7

Sap8

MKRPPGFSPFR

1318.7

 

MKRP(POH)GFSPFR

1334.7

 

(MO)KRPPGFSPFR

1334.7

 

(MO)KRP(POH)PGFSPFR

1350.7

 

RPPGFSPFR

1059.8

 

MKRPPGFSPF

1162.8

Sap9

MKRPPGFSPFR

1318.9

 

RPPGFSPFR

1059.6

 

PPGFSPFR

903.5

 

P(POH)GFSPFR

919.5

 

LMKRPPGFSPFR

1432.0

 

LMKRP(POH)GFSPFR

1447.8

 

PPGFSPF

747.4

  1. LK samples (1.5 μM) were digested with 0.03 μM Sap in citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (at a final concentration of 10 μM), and the samples were acidified with HCl (0.33 M) and subjected to LC-MS/MS analysis using the Bruker HCTultra ETDII IT mass spectrometer that was equipped with an ESI ion source and ETD II fragmentation module and was coupled to the Dionex Ultimate 3000 UHPLC system. MS/MS data were analyzed using in-house Mascot server (version 2.3.0), and the peptides were identified by searching the Swiss-Prot database. The peptides marked in bold generated peaks with the highest intensities (> 105 arbitrary units), which exceeded by ≥ 1 order of magnitude in comparison with the other peptides.