Figure 4From: Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans HPLC profiles of the fragmentation of LK by selected Saps. LK samples (1.5 μM) were digested with 0.03 μM of Sap1, −3, −4, −8, and −9 in the citrate buffer (pH 5.0) at 37°C for 24 hours. The reaction was stopped using pepstatin A (10 μM), followed by acidification with HCl (0.33 M). The samples were analyzed using HPLC on the Luna C18(2) 5 μm 4.6 × 250 mm column (Phenomenex) in a TFA water-ACN binary gradient system, as described in the Materials and Methods section. Arrows indicate the retention times of the Met-Lys-bradykinin (M) and des-Arg1-bradykinin (D) standards.Back to article page