Skip to main content

Table 4 Composition of media used in rMP1102 production assays

From: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33

 

Med-1 (Modified FM22)

Med-2

Med-3 (BSM)

Med-4

YPD

BMGY

KH2PO4

42.9

4

—

6

—

—

H3PO4

—

—

26.7 ml

—

—

—

(NH4)2SO4

5

4

—

—

—

—

NH4H2PO4

—

—

—

50

—

—

CaSO4

0.6

 

0.93

0.4

—

—

CaCl2

—

0.38

—

—

—

—

K2SO4

14.3

18.2

18.2

20

—

—

MgSO4•7H2O

11.7

9.4

14.9

15

—

—

KOH

—

—

4.13

1.5

—

—

Citric acid

1.92

—

—

—

—

—

Glucose

40

40

40

40

40

40

Peptone

—

—

—

—

20

20

Yeast extract

—

—

—

—

10

10

PTM1

0.48%

0.48%

0.48%

0.48%

0.48%

0.48%

Reference

[42]

[35]

[43]

[16]

—

—