Skip to main content

Table 3 Primers used in this study

From: The VieB auxiliary protein negatively regulates the VieSA signal transduction system in Vibrio cholerae

Primer Name

Primer Sequence 5′ − 3′

GST-VieS-C F

tcactgtgcatatgactgagcagctacgttggttgacgg

GST-VieS-C R

gagcgagtcggatcctcagagataacgactgagtactttgcgc

GST-VieS-C T2916C F

gttgattactgactgccacatgccacatcttgatg

GST-VieS-C T2916C R

catcaagatgtggcatgtggcagtcagtaatcaac

VieB F

tcactgtgcatatggctgtacctacttttgctgaattaaaag

VieB R

gagcgagtcggatccttacgcctcaactgattcgcttcgc

VieB D62A F

gatttgatatttttatttgcgcttacaacttcggtaaggggtt

VieB D62A R

aaccccttaccgaagttgtaagcgcaaataaaaatatcaaatc

VieB D62E F

gatttgatatttttatttgcgagtacaacttcggtaaggggtt

VieB D62E R

aaccccttaccgaagttgtactcgcaaataaaaatatcaaatc

MBP-VieS-C F

CGCGGATCCTTACGCAGCTCCGAACAAG

MBP-VieS-C R

ACGCGTCGACTTATTCGCTCTGATACTGATG

MBP-VieS-C D1018A F

TATGGCAATTGGTAATCAACAAATCATACTGCTCAGGATGTTGCGAGAGCTTTT

MBP-VieS-C D1018A R

CGAAAAGCTCTCGCAACATCCTGAGCAGTATGATTTGTTGATTACCAATTGCCA

  1. All primers are listed 5′ to 3′. Italics indicate restriction enzymes used for cloning.