Figure 5

Inhibition of 4 miRNAs after miRNA inhibitor treatment. Mock-infected group was inoculated with sterile PBS without any treatment. Mice in w81 and ma81 groups with no treatment were inoculated with w81 and ma81, respectively. Mice in treatment groups were transfected with the individual miRNA inhibitors (Bioneer Co. Ltd, Daejeon, Korea) or negative inhibitor control (miRNA negative inhibitor control #1; Bioneer Co. Ltd, Daejeon, Korea) as indicated, and then intranasally challenged with 5 MLD50 of ma81 H5N2 virus. RNA isolation in mouse lungs (n = 3/group) were conducted at 1 dpi. The miRNA expression levels were measured by qRT-PCR, and were normalized with RNU6. *, P value ≤0.05. Mock-infected: no treatment, then inoculated with sterile PBS. w81: no miRNA inhibitor, then inoculated with w81. ma81: no miRNA inhibitor, then inoculated with ma81. Negative inhibitor: miRNA inhibitor negative control #1 (Bioneer Co. Ltd, Daejeon, Korea) treatment, then inoculated with ma81. miRNA inhibitor: miRNA inhibitor (Bioneer Co. Ltd, Daejeon, Korea) treatment, then inoculated with ma81.