Figure 3From: Alcohol and tobacco consumption affects bacterial richness in oral cavity mucosa biofilms Rarefaction curves at two different OTU abundance thresholds. For each number of sequences the number of distinct OTUs was calculated; OTUs represented by one or two sequence reads were not considered. The distinct charts represent the rarefaction curves produced when we considered: (A) OTUs represented by at least 3 reads (or 0.03% of samples); (B) OTUs with a minimum of 100 reads (1%). Lines represent the average number of distinct OTUs ± standard error (S.E).Back to article page