Table 1 Microsatellite DNA sequences selected, sequences, and primers
From: Multilocus microsatellite markers for molecular typing of Candida tropicalis isolates
Microsatellite designation | Primer sequence | Repetitive motif | Range of PCR fragment size (bp) | Fluorescent label* |
|---|---|---|---|---|
Ctrm1 | F: CAACAGTTGATAGATCAAGC | (AGA) 22 | 370-454 | FAM |
R: CGAACTATCACTTTTAGGAG | ||||
ΔCtrm7 | F: GACTCTGAATCGGTTTTGTG | (CA)13 | 286-322 | HEX |
R: CGCTCATTCTCATAATCACT | ||||
Ctrm10 | F: AGTTTTCCTGTTGCTGGTTG | (ATG)52 | 315-370 | ROX |
R: CATTGAGATTGGAAGAAGTG | ||||
Ctrm12 | F: TGTGTGTCTATTACCTCCCA | (AC)39 | 234-263 | FAM |
R: CTGTCAGTTGTACATCATCG | ||||
ΔCtrm15N | F: CCCTACTAGGACCTCCACCG | (CAA)16 | 365-392 | TAMRA |
R: AAAGAATGGCGATGAAGTTG | ||||
Ctrm21 | F: TGTGTCTTGTAAAAGCCACC | (TG)22 | 328-363 | HEX |
R: GGATTACTGGACTTGACCTG | ||||
Ctrm24 | F: ACAACTACTGACATCCCAGC | (TA)13 | 439-454 | ROX |
R: CTTCAGTATTCACCCCTTTC | ||||
Ctrm28 | F: TAGTTCGAATTTGTTTGGAT | (TTA)12 | 397-406 | TAMRA |
R: GTAAAGTCACGGGGTATTGT |