Figure 5

L-Phe metabolic pathway analysis of K. apiculata by [2- ¹³ C] L-Phe labeling using GC-MS. A: Total ion chromatograph (TIC) of K. apiculata. (B-I) 13.097 min MS and (B-II) PEA standard MS. (C) Total ion chromatograph (TIC) of 2-phenylacetaldehyde (PAD; 10.09 min) and 2-phenylethanol (PEA;13.10 min) for different culture periods in dextrose minimal medium with [2-¹³C] L-Phe as the sole nitrogen source (0.76% YNB, 2% dextrose, 2% [2-¹³C] L-Phe). (1), (2), (3), (4) and (5) were 3 h, 6 h, 12 h, 24 h and 48 h analysis results after inoculation, respectively.