Figure 3
From: LM14 defined medium enables continuous growth of Trypanosoma cruzi
In vitro growth curve of T. cruzi in defined (LM14 and LM14B) and complex (LITB+FBS and LITB without FBS) media. Epimastigote cells counts were performed at every 24 hours, for eight days (beginning of cell death). Values plotted refer to the average of three biological replicates. Epimastigotes cultivated in defined medium LM14B (red line) had a similar profile of growth curve to parasites cultivated in LITB+FBS (black line), reaching the stationary phase at 5th day, as well as a similar growth curve was observed for epimastigotes cultivated in LM14 (green line) and LITB w/o FBS (dashed line).