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Figure 2 | BMC Microbiology

Figure 2

From: A non-pediocin low molecular weight antimicrobial peptide produced by Pediococcus pentosaceus strain IE-3 shows increased activity under reducing environment

Figure 2

Characterization of low molecular weight antimicrobial peptide produced by P. pentosaceus strain IE-3. (a) In-gel activity assay of crude extract on 18% tricine SDS-PAGE gel, lane 1 contains low molecular weight protein marker, lane 2 crude extract obtained from Diaion HP20 and lane 3 showing antimicrobial activity against L. monocytogenes MTCC 839 (b) Size determination by gel filtration chromatography of cation exchange purified peptide along with standard graph (of known molecular weight proteins depicts low molecular size). (c) Reverse-phase HPLC profile of purified antimicrobial peptide and inset showing the absorbance between 260–280 nm. (d) Intact molecular mass showing as 1701.00 Da in MALDI-TOF analysis.

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