Intracellular growth of Brucella in WT and Atg5
MEFs. MEFs were infected for 1h with B. abortus S2308 (A) or with B. melitensis 16M (B) at an MOI of 300. Log CFUs were obtained from cell lysates of infected WT MEFs and Atg5-/- MEFs at the indicated time after infection. Results represent means ± SD measured from at least three independent experiments made in triplicates. Statistical significance was calculated using the Holm-Sidak multiple comparisons test following a two-way ANOVA. p < 0.001 for both B. abortus and B. melitensis. *** indicates a highly significant difference using a Students t- test.