Table 4 Primers used in this study
From: Clostridium difficilehas a single sortase, SrtB, that can be inhibited by small-molecule inhibitors
Primer | Sequence |
|---|---|
pET_3 | gatattccatggatgaagaaactgtaccgtatcgttatc |
pET_4 | gatgagctcgaggatcagacgaccgtggataacc |
pET_17 | gatataccatggatgcaccaccaccaccaccactctaaactgaccaaatacaaccacgacac |
pET_16 | gatgagctcgagttagatcagacgaccgtggataac |
C209A | tcgttaccctgtctaccgccacctacgaattcgacg |
C209A_antisense | cgtcgaattcgtaggtggcggtagacagggtaacga |
T7F | taatacgactcactataggg |
T7R | gctagttattgctcagcgg |