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Table 2 FRET peptide details

From: Clostridium difficilehas a single sortase, SrtB, that can be inhibited by small-molecule inhibitors

Peptide sequence*

Description

d-IHSPSTGGG-e

Based on CD0183 sequence

d-IHGSSTPGG-e

Control for above peptide

d-SDSPKTGGG-e

Based on CD0386, CD3392 sequence

d-SDGSKTPGG-e

Control for above peptide

d-IHSPQTGGG-e

Based on CD2768 sequence

d-IHGSQTPGG-e

Control for above peptide

d-PVPPKTGGG-e

Based on CD2831 sequence

d-PVGPKTPGG-e

Control for above peptide

d-GQNVQTGGG-e

Based on CbpA sequence

d-QALPETGGG-e

SaSrtA peptide

d-NPQTN-e

SaSrtB peptide

d-IHSPSTGKT-e

Based on CD0183 sequence

d-SDSPKTGDN-e

Based on CD0386 sequence

d-IHSPQTGDV-e

Based on CD2768 sequence

d-PVPPKTGDS-e

Based on CD2831 sequence

  1. *Where d is Dabcyl (4-([4-(dimethylamino)phenyl]azo)-benzoyl) and e is Edans (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid).