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Table 4 Fold change in gene expression in histidine metabolism pathways

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene

Product

PM vs. WT 0

PM vs. WT 10

PM 0 vs. 10

PM 0 vs. 17.5

WT 0 vs. 10

  

ML

LL

ML

LL

ML

LL

ML

LL

ML

LL

Cthe_2880

ATP phosphoribosyltransferase regulatory subunit

98.42

29.12

98.72

80.51

1.25

1.01

1.12

−1.06

1.25

−2.73

Cthe_2881

ATP phosphoribosyltransferase

78.48

23.79

85.06

100.15

1.64

1.24

1.35

−1.01

1.52

−3.40

Cthe_2882

histidinol dehydrogenase

35.86

18.44

28.45

44.69

1.49

1.33

1.37

1.40

1.88

−1.83

Cthe_2883

histidinol-phosphate aminotransferase

38.12

19.61

23.12

40.22

1.15

1.22

1.19

1.42

1.89

−1.69

Cthe_2884

Imidazoleglycerol-phosphate dehydratase

7.45

7.71

17.31

17.09

1.23

1.25

1.18

1.27

−1.89

−1.77

Cthe_2886

Imidazole glycerol phosphate synthase subunit hisH

11.99

12.29

14.84

15.87

1.19

1.12

1.09

−1.01

−1.04

−1.16

Cthe_2887

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

13.46

11.01

10.02

14.54

1.44

1.20

1.29

1.13

1.93

−1.10

Cthe_2888

Imidazole glycerol phosphate synthase subunit hisF

12.46

14.23

10.04

18.19

1.61

1.30

1.54

1.24

1.99

1.02

Cthe_2889

Histidine biosynthesis bifunctional protein hisIE

12.71

10.80

6.09

12.49

1.48

1.29

1.51

1.28

3.08

1.11

Cthe_3028

Pyridoxal-dependent decarboxylase

−11.35

−13.46

−7.10

−6.92

−2.37

−1.04

−3.78

−2.89

−3.79

−2.02

Cthe_3149

aminoacyl-histidine dipeptidase

3.34

4.23

−1.07

1.63

1.15

1.05

1.39

1.37

4.09

2.72

Cthe_1332

Histidyl-tRNA synthetase

−1.58

−1.89

1.66

−1.18

1.10

−1.03

−1.15

−1.62

−2.38

−1.64

  1. Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.