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Table 2 Fold change in gene expression along the central metabolism and mixed-acid fermentation pathways

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene

Product

PM vs. WT 0

PM vs. WT 10

PM 0 vs. 10

PM 0 vs. 17.5

WT 0 vs. 10

  

ML

LL

ML

LL

ML

LL

ML

LL

ML

LL

glucose-6-phosphate to PEP

Cthe_0347

Phosphofructokinase

1.77

2.59

2.97

2.13

−1.35

−1.31

−1.14

−1.49

−2.27

−1.07

Cthe_0349

fructose-1,6-bisphosphate aldolase, class II

1.60

2.49

3.31

2.50

−1.52

−1.41

−1.49

−2.03

−3.14

−1.42

Cthe_2449

Phosphoglycerate mutase

−2.46

−1.85

1.42

−1.74

−1.48

−1.90

−2.01

−2.88

−5.18

−2.03

Cthe_3153

alpha-ribazole phosphatase

2.11

2.33

1.40

1.40

1.21

1.23

1.42

−1.14

1.82

2.04

Cthe_0143

Enolase

−1.23

−1.04

1.63

−1.02

−1.11

−1.13

−1.05

−2.96

−2.22

−1.16

Non-oxidative Pentose Phosphate pathway

Cthe_2443

Transketolase domain-containing protein

−3.24

−4.70

1.02

−3.00

1.14

−1.75

−1.90

−1.52

−2.88

−2.74

Cthe_2444

Transketolase domain-containing protein

−3.47

−4.63

−1.15

−3.39

1.26

−1.72

−1.63

−1.57

−2.41

−2.36

Cthe_2705

Transketolase central region

−1.44

−1.33

2.25

1.19

1.24

1.21

1.17

−1.81

−2.60

−1.32

PEP to Pyruvate

Cthe_2874

Phosphoenolpyruvate carboxykinase [GTP]

1.39

2.46

1.43

2.09

−1.05

−1.04

1.30

1.38

−1.07

1.14

Cthe_0344

malic protein NAD-binding

−1.68

1.06

1.26

−1.10

−1.01

−1.14

1.20

−1.27

−2.13

1.02

Cthe_1308

pyruvate, phosphate dikinase

1.64

2.29

−1.30

1.65

−1.05

1.07

1.30

1.10

2.03

1.49

Pyruvate to Lactate/Formate/Acetyl-CoA

Cthe_1053

L-lactate dehydrogenase

−1.78

−1.25

1.32

−1.02

−1.41

−1.27

−1.33

−1.16

−3.30

−1.55

Cthe_2794

pyruvate/ketoisovalerate oxidoreductase, gamma subunit

4.30

1.48

5.15

3.99

1.92

2.56

2.45

2.78

1.61

−1.05

Cthe_2796

pyruvate flavodoxin/ferredoxin oxidoreductase domain protein

3.13

1.47

4.16

2.94

1.98

2.05

1.88

1.94

1.49

1.02

Cthe_0505

formate acetyltransferase

−1.95

−1.91

1.46

−1.04

1.24

1.04

1.11

−1.81

−2.31

−1.76

Acetyl-CoA to Ethanol/Acetate

Cthe_1028

Acetate kinase

1.67

2.57

3.63

3.05

2.12

1.26

2.76

1.50

−1.02

1.06

Cthe_1029

phosphate acetyltransferase

1.54

1.79

4.01

3.83

2.42

1.33

2.73

1.63

−1.08

−1.61

Cthe_2238

Aldehyde Dehydrogenase

1.06

1.04

−1.81

−1.29

1.20

1.36

1.36

1.02

2.30

1.83

Cthe_0101

iron-containing alcohol dehydrogenase

−1.35

−1.19

2.19

1.20

1.22

−1.04

−1.18

−1.82

−2.43

−1.48

Cthe_0423

iron-containing alcohol dehydrogenase

1.12

1.07

4.75

5.02

1.26

1.06

1.28

1.45

−3.36

−4.42

  1. Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.