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Table 1 Fold change in expression of sigma factors

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene name

Product

PM vs. WT 0

PM vs. WT 10

PM 0 vs. 10

PM 0 vs. 17.5

WT 0 vs. 10

  

ML

LL

ML

LL

ML

LL

ML

LL

ML

LL

Cthe_1272

sigma-70 region 2 domain protein

2.34

1.24

−5.64

−3.59

−2.20

−1.64

−1.38

1.94

6.00

2.72

Cthe_0195

Sigma-70 region 4 type 2

2.80

1.61

−2.48

−1.42

−2.06

−1.23

−1.44

1.49

3.37

1.86

Cthe_1438

RNA polymerase sigma factor, sigma-70 family

2.68

2.06

1.70

−1.38

−2.26

−1.76

−2.95

−2.42

−1.43

1.61

Cthe_0890

RNA polymerase sigma factor, sigma-70 family

−1.09

−1.63

−2.01

−1.12

1.45

−1.64

−1.27

−1.14

−1.13

1.21

Cthe_1809

RNA polymerase sigma factor, sigma-70 family

18.26

16.44

24.37

13.05

−1.69

−2.11

−4.55

−4.06

−2.25

−1.68

Cthe_0446

sigma-E processing peptidase SpoIIGA

−1.86

−2.21

−1.14

1.26

−1.10

1.45

−1.03

1.51

−1.78

−1.92

Cthe_0447

RNA polymerase sigma-E factor

1.90

2.58

2.15

1.91

−1.56

−1.19

−1.30

−2.65

−1.77

1.14

Cthe_0120

RNA polymerase sigma-F factor

1.71

2.01

2.48

1.96

1.01

1.15

−1.03

−1.22

−1.43

1.18

Cthe_0448

RNA polymerase sigma-G factor

−1.79

−2.55

1.09

−1.14

−2.10

−1.23

−1.56

−1.06

−4.11

−2.73

Cthe_1012

RNA polymerase sigma-K factor

−3.94

−4.74

−2.88

−2.96

1.13

1.20

1.07

3.57

−1.21

−1.33

Cthe_2059

RNA polymerase sigma-H factor

1.45

1.65

1.86

1.03

−1.30

−1.52

−1.41

−2.13

−1.66

1.05

Cthe_0074

RNA polymerase, sigma-24 subunit, ECF subfamily

−1.19

−1.46

−1.87

−2.22

3.64

1.40

3.54

1.74

5.71

2.13

Cthe_0495

RNA polymerase, sigma 28 subunit

−3.04

−3.47

−9.98

−4.44

1.18

1.43

1.37

1.53

3.87

1.83

Cthe_2100

transcriptional regulator, AbrB family

2.21

2.48

8.86

1.29

−2.67

−1.16

−5.28

−13.66

−10.68

1.66

Cthe_0315

RNA polymerase sigma-I factor

−1.40

−2.19

−4.00

−3.49

−1.13

−1.17

−1.00

1.92

2.52

1.36

Cthe_2975

RNA polymerase sigma-I factor

1.24

1.47

−7.26

−2.59

−2.09

−1.94

1.15

1.45

4.32

1.96

Cthe_0403

RNA polymerase sigma-I factor

−1.65

−1.92

−5.17

−3.64

1.89

1.76

−1.66

−1.08

−1.12

1.83

  1. Bold values indicate significantly different expression levels as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate a positive/negative value represents a higher/lower level of expression in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) a positive/negative value represents a higher/lower expression in the hydrolysate media compared to standard medium. Values are indicated for samples collected during the mid-log (ML) and late-log (LL) growth phases.