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Figure 4 | BMC Microbiology

Figure 4

From: Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection

Figure 4

Cellular and molecular responses of osteoblasts and macrophages infected with S. aureus at an MOI of 500:1 for 2 h. (A) DCF fluorescence intensity as an indicator of H2O2 production by non-infected controls and S. aureus-infected osteoblasts and macrophages. (B) DHE fluorescence intensity as an indicator of O. 2 − production by non-infected controls and S. aureus-infected osteoblasts and macrophages. (C) Osteoblast ALP activity measured at post-infection days 1, 4, and 7. (D) Macrophage phagocytosis activity (ingestion). Light gray area: control macrophages without incubation with S. aureus; dark gray area: non-infected macrophages; black area: infected macrophages. * p < 0.01, ** p < 0.001, *** p < 0.0001, and # p < 0.05 compared to control.

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