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Figure 5 | BMC Microbiology

Figure 5

From: Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon

Figure 5

Analysis of BsaN box requirements for transcription activation by BsaN/BicA. The ability of BsaN/BicA to directly activate the expression of truncated promoters was examined by providing regulatory genes in trans and measuring β-galactosidase activities arising from the expression of transcriptional promoter-lacZ fusions in E. coli DH5α. The top sequence of each gene includes the intact promoter region; sequence 1 is deleted up to the BsaN box; sequence 2 also includes a 6 nucleotide deletion of the BsaN box. Effect of BsaN/BicA on the expression of A. PbicA-lacZ fusion, B. PvirA-lacZ fusion and C. Ps1518-lacZ fusion; Ps1518 denotes the promoter region of BPSS1518. Effect of BsaN/BicA on the expression of D. PbprD-lacZ fusion and E. PbopA-lacZ fusion.

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