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Figure 1 | BMC Microbiology

Figure 1

From: Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon

Figure 1

Regulation of selected genes by BsaN as analyzed by RNAseq and qRT-PCR. A. Activation and repression of T3SS3 cluster genes as analyzed by RNAseq. The adjusted p value for all genes is less than 0.01 with the exception of three genes denoted with ^. B. Activation of BsaN regulated T6SS1 and bim motility genes as analyzed by RNAseq. C and D qRT-PCR validation of selected activated genes. Expression of each in wild-type B. pseudomallei KHW gene is set to 1; transcription was normalized to that of the recA reference gene. E. qRT-PCR validation of repressed genes. Expression of each in wild-type B. pseudomallei KHW gene is set to 1; transcription was normalized to that of the 16S rRNA reference gene. The flgL gene is located upstream and in the same transcriptional unit as flgK.

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