Table 5 Primers used for the screening of pRS218 genes among neonatal meningitis causing E. coli and fecal commensal E. coli strains

4107- 4265

pRS218_007

Copper sensitivity

gagacgttgagcaccaatctg

accgccagtttttctttcac

140

4255- 4761

pRS218_008

Copper sensitivity

catacgctggacggggaaac

gacgctctccccttccgact

143

4998- 5759

pRS218_010

Na + traslocation

atcaatgatggtgctttgtgtc

ccggtaactggaatgataacct

378

6052-7992

pRS218_013

Iron permease

gtgttcgagaacctggaagg

cggttttgtctgagggacat

401

8033- 8560

pRS218_014

Iron transport

ctgtcaccatgaatgaaatgga

ctcacatcaaacggtttccac

400

8664- 10043

pRS218_015

Membrane protein

tcgtgacggtaaactgcatc

gccgccatagctgtatttgt

400

10046- 11329

pRS218_016

ABC transporter

aaggggtggtgatcgataaaat

catacagcacctccacaggata

399

11319- 12449

pRS218_017

Membrane protein

aggtcaccggtagctggatt

atcgagaccagtcccatcag

400

12454- 13149

pRS218_018

ABC transporter

gttccatttgatcccgttctta

acccagatatttaccgtgttgc

379

13136- 13621

pRS218_019

Putative thioredoxin precursor

gcgggtgtaaagaagaaaagc

agacggcttacgcataccc

401

13646- 14131

pRS218_020

Hypothetical protein

atagcgcaactgcttcacacta

acgttccgtatcgacaaattct

303

14253- 14702

pRS218_022

Glucose-1-phosphatase

agacaacgccggaaggttat

tttcctgatgatgtaccggaat

354

14677- 14997

pRS218_023

Glucose-1-phosphatase

acgatggacccaacgtttaat

ataggctgattcgatgtgtttg

311

18173- 17826

pRS218_031

Hypothetical protein

attgccctgatggacagc

gtggcagccggttaacttt

301

20251-20775

pRS218_034

Colicin immunity

ttaataatatgtggtggggatgg

atgaaaacagtacccgtataaacagc

250

21065-21982

pRS218_035

ColicinJ production

tggcttattcaaaatttgctcat

tgcatagatatgatggtttcacg

350

21990-22766

pRS218_036

ColicinJ production

ctgattttccttgcgtttatctg

agcctttatcttacgaggtggac

294

22935- 25196

pRS218_038

ColicinJ production

tatgatgcaggttttgcttttg

tggcatcatgttgagcttattc

393

25265- 26440

pRS218_039

Enterotoxin

gcagattcgcgttttgagca

cggatctttcaacgggatgg

302

28517- 27762

pRS218_042

Hypothetical protein

tgacgctatgcaatgaagaact

tgacatagccaagatcatccac

399

38291- 37500

pRS218_056

Hypothetical protein

cgtccacggattatgtctataaaac

gtatgacgggatgatttcagataac

373

40184- 38298

pRS218_057

ColicinJ production

ctgtggataacagcctcatcaa

atgttaaccgggtagcttttca

301

43799-42630

pRS218_060

Hypothetical protein

ctcttccccatggcctttat

accccatactgcattggaaa

600

46748- 46975

pRS218_063

Hypothetical protein

tggatcctttgttgatcattcat

cctgtaaagacagacttcagaaaaa

224

48251- 47610

pRS218_064

Hypothetical protein

tcgacctaacccttgatcagtt

tatagcgacaggatggacagtg

385

52321- 52046

pRS218_073

Hypothetical protein

cagccagcaagcattaaaca

gctcaagggctactctgacg

276

53188- 54159

pRS218_074

Stability protein StbA

ttgtcgcaaaactcatttcg

cgaccagacgagaaaacaca

400

56513- 56265

pRS218_079

Hypothetical protein

cgcattgaaattcttttcgac

tcgtcctgccagatttcttc

249

56648- 57166

pRS218_080

Unknown

gtgttcgtgatctcgtttcgta

ttgcccactttcttaatcttcc

351

58824- 59654

pRS218_082

Hypothetical protein

acaaatgaaggtattcagctgtttc

cgacagtacgttgtcacacagac

372

60445-59648

pRS218_083

Transposase

gcttcgggaacgctgtaacg

agaaggctgcggtgctgaag

414

61858- 62169

pRS218_086

Hypothetical protein

ttttccggtaaaggatgtcg

gtctttctgacggcaaggctat

223

62245- 62928

pRS218_088

Adenine-specific methyltransferase

cggtgatgttaatgatgactgg

gtgtgaagctctcaatcagtgg

356

62929- 63150

pRS218_089

Cytoplasmic protein

ctatgccggacacgaaaaac

gaagcaggaatccagttcca

208

63230- 63598

pRS218_090

Hypothetical protein

gttatctggtccccggaaga

cattcacgtttccacaatgc

254

63643- 64614

pRS218_091

Hypothetical protein

atgaatgaaatgctgaatgcac

catcttctgccacctggtaact

406

63643-64614

pRS218_091

Hypothetical protein

cgcctggtggtgaaggaaag

gaccacctcccgcagaacac

236

64828- 65253

pRS218_092

Putative antirestriction protein

gttgaagagtgcgaccgtct

agtcaagtgccgcgtaaatc

400

65300- 65722

pRS218_093

Phage protein MubC

catccgcgatgtactggatac

ctgtaacacaacgtccattgct

373

65719- 65910

pRS218_094

Hypothetical protein

cacagaaacccgcgaaat

ctgtttctgctgccctgtaag

177

66381- 65887

pRS218_095

Hypothetical protein

cttacatcccggcgtcgt

cctgatgttatgtttctgtggttact

256

67155- 68516

pRS218_099

Hypothetical protein

tatggcaaaactcatcagcagt

gtaatttggcgttgtgactgaa

385

68563- 69126

pRS218_100

Hypothetical protein

tctcagctttttgtgagtcctg

aaaacggtaacagcttctcctg

400

70556- 70789

pRS218_105

Cytoplasmic protein

gcgaatatttcagaatacttcagg

aattccggatgacatggttc

213

70848- 72806

pRS218_106

Hypothetical protein

agtgtgaggaatctgacctgct

taatgtttacattccaggctgattt

400

72861- 73271

pRS218_107

Adenine-specific methyltransferase

ataccatgaacgcacaggaata

ggatgatgtcgttaacgctgta

371

74286- 74444

pRS218_109

Hok/Gef cell toxic protein

atgaaactaccacgcagctctc

taccggattcgtaagccatga

154

75004- 74681

pRS218_110

Hypothetical protein

gcgttgcgccttacatcc

tcacatcaccttccctttgatt

314

75360- 75647

pRS218_113

Hypothetical protein

gagtacccgaaatatccacgtt

taatctgacgcaggaactgttt

251

75360-75647

pRS218_113

Hypothetical protein

tgggggctgaaaaccagaga

accgaaggcacgaactgcat

531

75691- 76587

pRS218_114

Unknown

tcggtattttccggtgataaac

ataacctgcccgacaatatcac

359

77473- 76883

pRS218_116

X polypeptide

aggccgggattacaaaatagat

ccggtataaatccggtaaacct

354

78394 79080

pRS218_118

TraJ/conjugal transfer

caatggggcttttattgaactc

tgaccaacacccagcatataaa

369

85396- 85614

pRS218_131

Hypothetical protein

tgcatacctttatttttcttgtgc

tcagtgtatccatcacgttgttc

210

89620-90612

pRS218_136

TraU/conjugal transfer

ttccttctcgccggtcatgt

ccagcgagagcgggaaaata

111

105274- 110544

pRS218_154

TraI/conjugal transfer

gcgatgcggtcagtgttctg

ggacagccgttcatcctgct

190

111369- 112229

pRS218_156

Dienelactone hydrolase

tctggttaccggagagatgaat

agtaccagaagcaacagcatca

343

113415- 113939

pRS218_159

Hypothetical protein

gtgccatttatctgatatggagaat

tctgtgttgtactgctcatataccc

387

113985- 114194

pRS218_190

Hemolysin expression modulating protein

caaaacaggaatggctgtatca

tatttccatatctcttttggtatcctg

190