Figure 7

HmuR mutant of P. gingivalis is deficient in community accumulation. A) Confocal microscopy showing x-y and x-z projections of communities of S. gordonii (red), F. nucleatum (green) and P. gingivalis (blue) wild type (WT) or ΔhmuR mutant strains. Representative image from three independent experiments. B) Confocal microscopy showing x-y and x-z projections of single species P. gingivalis WT or ΔhmuR mutant accumulations. Representative image from three independent experiments. C) Biovolume analysis of P. gingivalis WT or ΔhmuR mutant accumulation in the P. gingivalis-F. nucleatum-S. gordonii communities shown in A. D) Biovolume analysis of P. gingivalis WT or ΔhmuR single species accumulations shown in B. E) Biomass of P. gingivalis WT or ΔhmuR single species accumulations measured by crystal violet staining and release. F) Biovolume analysis of P. gingivalis WT or ΔhmuR accumulation in two species P. gingivalis-S. gordonii communities. G) Biomass of P. gingivalis WT or ΔhmuR two species accumulation with F. nucleatum measured with P. gingivalis antibodies. ** denotes p < 0.01 (n = 3) compared to WT.