Figure 3
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD , wbkF , wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Agfrom B. melitensis 16 M uncut; 3, manAO-Agfrom B. melitensis 16 M cut by Ava II; 4, manAO-Agfrom B. neotomae cut by Ava II; 5, wbkF from B. melitensis 16 M uncut; 6, wbkF from B. melitensis 16 M cut by Alu I; 7, wbkF from B. melitensis bv2 cut by Alu I; 8, wbkF from B. abortus bv2 cut by Alu I; 9, wbkF 2* from B. melitensis 16 M uncut; 10, wbkF 2* from B. canis uncut; 11, wbkF 2* from B. melitensis 16 M cut by EcoR V; 12, wbkF 2* from B. canis cut by EcoR V; 13, wboA from B. melitensis 16 M uncut; 14, wboA from B. melitensis 16 M cut by Alu I; 15, wboA from B. abortus cut by Alu I; 16, wa** from B. melitensis 16 M uncut; 17, wa** from B. melitensis 16 M cut by Ava II; 18, wa** from B. suis bv2 cut by Ava II; 19, wa** from B. melitensis 16 M cut by Hinf I; 20, wa** from B. ovis cut by Hinf I. Panel B. Lanes: 1, molecular size markers; 2, manBO-Agfrom B. melitensis 16 M uncut; 3, manBO-Agfrom B. pinnipedialis uncut; 4, manBO-Agfrom B. melitensis 16 M cut by Sau 3A; 5, manBO-Agfrom B. melitensis bv2 cut by Sau 3A; 6, manBO-Agfrom B. abortus cut by Sau 3A; 7, manBO-Agfrom B. suis cut by Sau 3A; 8, manBO-Agfrom B. suis bv2 cut by Sau 3A; 9, manBO-Agfrom B. ovis cut by Sau 3A; 10, manBO-Agfrom B. pinnipedialis cut by Sau 3A; 11, manBO-Agfrom B. ceti cut by Sau 3A; 12, manBO-Agfrom B. melitensis 16 M cut by Eco RV; 13, manBO-Agfrom B. abortus cut by Eco RV. Panel C. Lanes: 1, molecular size markers; 2, wbkD from B. melitensis 16 M uncut; 3, wbkD from B. abortus uncut; 4, wbkD from B. canis uncut; 5, wbkD from B. melitensis 16 M cut by Sau 3A; 6, wbkD from B. abortus cut by Sau 3A; 7, wbkD from B. canis cut by Sau 3A.