Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella

BMC Microbiology

Table 3 Results obtained by the real-time PCR and the Salmonella BAX PCR in the external validation.

*Salmonella*level (CFU/25-g sample)	No. of samples analyzed	Result obtained by the PCR and BAX methods^a
		PA	PD	ND	NA	Inconc./+
1000	3	3	0	0	0	0
100	3	3	0	0	0	0
10	9	7	0	0	2	0
5	12	10	1	0	0	1
2	12	9	0	1	2	0
TOTAL	39	32	1	1	4	1

^a PA: positive by PCR and BAX methods, PD: positive by PCR and negative by BAX, ND: negative by PCR and positive by BAX, NA: negative by PCR and BAX methods, inconc./+: inconclusive result by PCR (need re-analysis) and positive by BAX.

ISSN: 1471-2180