Figure 4
From: piggyBac is an effective tool for functional analysis of the Plasmodium falciparumgenome
piggyBac inserts into AT-rich regions of the P. falciparum genome. (a) Nucleotide composition analysis of the flanking sequences showed that piggyBac inserted TTAA sites preferentially occur in the middle of an AT-rich core of 10 nucleotides predominantly with 'T's upstream (χ2 test, df 1, P = 6.3 × 10-5) and 'A's downstream (χ2 test, df 1, P = 2.07 × 10-8) as compared to randomly selected genomic TTAA sequences. (b) A comparison of nucleotide composition of flanking sequences only in the 5' untranslated regions (UTRs) of piggyBac inserted and randomly selected TTAA sequences further confirms the specificity of piggyBac for AT-rich target sites.